Selected Methods of Instrumental Analysis   (351H2)

Academic year:

2023/2024.

Attendance requirements:

There are no requirements.

ECTS:

9

Study level:

Study programs:

Teachers:

Rada M. Baošić, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Tatjana Ž. Verbić, Ph.D.
associate professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Aleksandar Đ. Lolić, Ph.D.
associate professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

Miloš P. Pešić, Ph.D.
assistant with PhD, Faculty of Chemistry, Studentski trg 12-16, Beograd

Olivera S. Marković, Ph.D.
scientific associate, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, Beograd

Hours of instruction:

Weekly: four hours of lectures + two hours of exercises + three hours of labwork (4+2+3)

Goals:

Students will gain and/or complete their knowledge in various instrumental techniques used in analytical, environmental, pharmaceutical and medicinal chemistry.

Outcome:

Students will be qualified for independent research, optimization and sensitivity improvement of various instrumental methods for the analysis of complex samples.

Teaching methods:

Lectures, theoretical exercises, lab work, semester paper.

Extracurricular activities:

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Coursebooks:

Main coursebooks:

1. J. A. C. Broekaert: Analytic Atomic Spectroscopy with Flames and Plasmas, John Wiley & Sons, Inc., Hoboken, New Jersey, USA.
2. J. Wang: Analytical Electrochemistry, John Wiley & Sons, Inc., Hoboken, New Jersey, USA.
3. A. J. Bard, L. R. Faulkner: Electrochemical methods, fundamentals and application, John Wiley & Sons, Inc., Hoboken, New Jersey, USA.
4. J. Ruzicka, E. Hansen: Flow Injection Analysis, Wiley Interscience, John Wiley & Sons, Inc., Hoboken, New Jersey, USA.
5. F. Settle: Handbook of instrumental techniques for analytical chemistry, Prentice Hall PTR, Upper Saddle River, NJ 07458
6. J. R. Lakowicz: Principles of Fluorescence Spectroscopy, Springer Science Business Media, New York, USA.
7. Z. Mandić (editor): Physico-Chemical Methods in Drug Discovery and Development, IAPC Publishing, Zagreb, Croatia, 2012.

Supplementary coursebooks:

• T. Verbić, M. Pešić, N. Obradović, I. Cvijetić: Praktikum sa zbirkom zadataka iz instrumentalne analitičke hemije - optičke i elektroanalitičke metode, Univerzitet u Beogradu - Hemijski fakultet, 2018.

Lectures and manuals for lab work will be provided during the course through Faculty network.

Additional material:

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Lectures:

5 points (4 hours a week)

Syllabus:

1. Modern optical methods, introduction.
2. Atomic absorption and emission spectroscopy.
3. Flow-injection analysis.
4. Spectroelectrochemistry.
5. Cyclic and stripping voltammetry.
6. Pulse voltammetric techniques.
7. Electrochemical sensors and electrode modifications.
8. Electrochemical methods of separation.
9. Hybrid techniques.
10. Application of instrumental methods in environmental chemistry.
11. Potentiometry, potentiometric titrations and potentiometric biosensors.
12. Fluorescence spectroscopy (FS) - principles and application.
13. Capillary electrophoresis (CE) - principles and application.
14. Detection methods in liquid chromatography.
15. Equilibria in analytical chemistry - methods for determination of dissociation and stability constants, lipophilicity and solubility.
16. Experimental techniques for measurements of plasma proteins - small molecules binding.
17. Isothermal titration calorimetry (ITC) - principles and application.
18. Surface plasmon resonance (SPR) - principles and application.
19. źolecularly imprinted polymers (MIPs).

Exercises:

0 points (2 hours a week)

Syllabus:

Theoretical exercises are combined with lab work. During the long-lasting experiments attention will be paid to particular case studies related to the on-going thematic unit addressed during lectures and laboratory exercises.

Labwork:

20 points (3 hours a week)

Syllabus:

1. Matrix effect on metal determination by flame atomic absorption spectrometry.
2. Determination of heavy metals by electrothermal atomic absorption spectrometry.
3. Determination of ascorbic acid by cyclic voltammetry in fresh fruits and vitamin C tablets.
4. Determination of ascorbic acid by flow injection analysis.
5. Determination of metals by flow injection analysis on modified carbon electrode.
6. Determination of diazepam on modified screen-printed electrode.
7. Ion-selective elecrodes: application of nitrate-selective and iodide-selective electrodes in real samples analysis. Sensors for oxygen and carbon dioxide.
8. Fluorescence spectroscopy: examination of small biologically active molecule – HSA interactions.
9. Chromatographic examination of propranolol binding to AGP. Separation of propranolol enantiomers on chiral HPLC column.
10. Fluorescence polarization: determination of progesteron in milk by immunoassay.
11. Molecularly imprinted polymers: conductometric study of monomer–template interactions in prepolymerization mixture. Preparation of molecularly imprinted (MIP) and non-imprinted polymer (NIP).
12. Molecularly imprinted polymers: study of template binding to MIP and NIP by HPLC. NMR spectroscopic study of the template–polymer interactions.

Semester papers:

20 points

Oral exam:

55 points