High Throughput Screening of Biocatalysts   (484B2)

Academic year:

2023/2024.

Attendance requirements:

There are no requirements.

ECTS:

10

Study level:

Study programs:

Teacher:

Radivoje M. Prodanović, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: five hours of lectures + three hours of study research work

Goals:

Introducing students to the state of the art techniques of analytical biochemistry for finding biocatalysts with high-throughput.

Outcome:

Know-how for design of high-throughput screening systems for biocatalysts.

Teaching methods:

Lectures, semmester papers.

Extracurricular activities:

—

Coursebooks:

• William P. Janzen: High Throughput Screening - Methods and Protocols, Humana Press, 2004.
• Jean-Louis Reymond: ľnzyme Assays - High-throughput Screening, Genetic selection and Fingerprintig, Wiley-VCH 2006.
• Jean Berthier, Pascal Silberzan: Microfluidics for Biotechnology, Artech House, 2006.
• Paul C. H. Li: Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery, Taylor & Francis, 2006.
• Selected review papers

Additional material:

—

Lectures:

10 points (5 hours a week)

Syllabus:

1. Principles for design of enzymatic assays in micro-titer plates and micro-compartments.
2. Robotic and automatic systems for liquid handling.
3. In vitro compartmentalization in high-throughput screening.
4. Biocatalysts screening using flow cytometry (FACS).
5. Microfluidics and development of lab on chip devices for biocatalysts screening.
6. Screening methods based on genetic selection and genetic circuits.
7. High-throughput screening of metagenomic libraries for biocatalysts.
8. High-throughput screening methods in directed evolution of enzymes and protein engineering.

Semester papers:

40 points

Oral exam:

50 points

Study research work:

0 points (3 hours a week)