Course code:
421B1		 Course name:
Immunochemistry

Academic year:

2019/2020.

Attendance requirements:

401B1 + 404B1

ECTS:

7

Study level:

basic academic studies

Study program:

Biochemistry: 4. year, winter semester, compulsory course

Teacher:

Marija Đ. Gavrović Jankulović, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistant:

Isidora I. Protić Rosić, Ph.D.
teaching assistant, Faculty of Chemistry, Studentski trg 12-16, Beograd

Hours of instruction:

Weekly: two hours of lectures + four hours of labwork (2+0+4)

Goals:

The goal of this course is to help students acquire the basic knowledge in the field of immunochemistry.

Outcome:

At the end of this course students will be familiar with the basic principles of the cellular and humoral immune response. Students will also master the techniques which are based on the antigen-antibody interaction through experimental work.

Teaching methods:

Lectures, experiments, term papers.

Extracurricular activities:

Preparing one’s term paper.

Coursebooks:
  • Roitt, Brostoff, Male, Mosby: Immunology, 6th edition

Additional material:

—

  Course activities and grading method

Lectures:

10 points (2 hours a week)

Syllabus:

  • Introduction to immunochemistry.
  • The structure and function of immunoglobulins.
  • The immunological diversity.
  • Basic principles of immunochemical techniques.
  • Human leukocyte antigens. Antigen presentation. T-cell receptors.
  • Maturation, activation and differentiation of T cells.
  • Maturation, activation and differentiation of B cells.
  • Complement.
  • Cell cooperation in the immune response.
  • Regulation of the immune response.
  • The types of hypersensitivity.
  • Tumor immunology.
  • Autoimmunity.

Labwork:

15 points (4 hours a week)

Syllabus:

  • Production of antisera (immunization, bleeding) and testing the quality of the antisera (immunodiffusion and immunoelectrophoresis techniques).
  • Preparation of an immunoaffinity matrix and isolation of antigens by immunoaffinity chromatography.
  • The immunoblot.
  • Enzyme-linked immunosorbent assay (ELISA).
  • Functional assays for the determination of the activation of the complement system.
  • Proliferation of T cells.
  • The application of fluorescence-activated cell sorting (FACS) in determination of surface markers of T cells.

Semester papers:

10 points

Colloquia:

15 points

Oral exam:

50 points