Course code:
373H2		 Course name:
Modern Chromatographic Methods

Academic year:

2023/2024.

Attendance requirements:

There are no requirements.

ECTS:

10

Study level:

doctoral academic studies

Study programs:

Chemistry: 1. year, winter semester, elective (E72H2) course

Chemistry: 1. year, winter semester, elective (E71H2) course

Chemistry: 2. year, winter semester, elective (E74H2) course

Chemistry: 2. year, winter semester, elective (E73H2) course

Teacher:

Dušanka M. Milojković Opsenica, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: five hours of lectures + three hours of study research work

Goals:

The main aim of the course is to provide a higher level of knowledge regarding to chromatographic methods that will be able to apply in the preparation of his doctoral dissertation and further scientific research work. Students will be also introduced to the latest trends in planar and column chromatography.

Outcome:

Students will learn how to select, optimize, and apply chromatographic techniques for both identification and quantification of various compounds from complex samples.

Teaching methods:

Lectures, semester papers.

Extracurricular activities:

—

Coursebooks:

Main coursebooks:

  1. V. R. Meyer: Practical High-Performance Liquid Chromatography, John Wiley, 2000.
  2. M. Holčapek, W. C. Byrdwell (eds.): Handbook of Advanced Chromatography / Mass spectrometry techniques, Academic press, Elsevier, London, 2017.
  3. T. Kowalska, M. Sajewicz, J. Sherma (eds.): Planar Chromatography - Mass Spectrometry, CRC Press, Taylor & Francis Group Inc., New York, 2015.
  4. C. Poole (ed.): Instrumental Thin-Layer Chromatography, Handbooks in Separation Science, Elsevier Science, Amsterdam, 1st edition, 2014.

Supplementary coursebooks:

  • Lecture handouts.

Additional material:

—

  Course activities and grading method

Lectures:

10 points (5 hours a week)

Syllabus:

  1. History and development of chromatographic techniques, basic concepts and elements of advanced conteprorary chromatography.
  2. High performance and ultra high performance liquid chromatography coupled with NMR and high resolution mass spectrometry (HRMS).
  3. Novel materials and stationary phases in liquid chromatography.
  4. Chromatographic mechanisms of separation.
  5. Supercritical chromatography, hydrophilic interaction chromatography and separation of chiral compounds.
  6. Miniaturzation of chromatographic systems in liquid column chromatography.
  7. Multiple dimensions in liquid chromatography.
  8. HPLC-based activity profiling.
  9. Scrining of bilologically active compounds by high-performance thin-layer chromatography (antimicrobial, antioxidative, estrogenic, enzymtic and immunological bioatugraphy assays).
  10. High performance thin layer chromatography coupled to mass spectrometry (HPTLC-UV / Vis / FLD-HRMS, HPTLC-UV / Vis / FLD-DART-HRMS, HPTLC-UV / Vis / FLD-DESI-HRMS).
  11. Miniaturization in planar chromatography.

Semester papers:

40 points

Oral exam:

50 points

Study research work:

0 points (3 hours a week)