Course code:
312A2		 Course name:
Separation Methods

Academic year:

2024/2025.

Attendance requirements:

302H2 / 302A2 / 301B2

ECTS:

8

Study level:

basic academic studies, integrated basic and graduate academic studies

Study programs:

Chemistry: 3. year, summer semester, elective (E13H2) course

Biochemistry: 3. year, summer semester, elective (E13B2) course

Chemical Education: 3. year, summer semester, elective (E14P2) course

Teacher:

Maja M. Natiæ, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: four hours of lectures + two hours of labwork (4+0+2)

Goals:

Within this course students are introduced to the basic principles of separation methods and the ways of selecting the appropriate method depending on the needs.

Outcome:

A student is able to select and apply the most suitable method for the appropriate separation.

Teaching methods:

Lectures, labwork, term paper.

Extracurricular activities:

—

Coursebooks:
  • C. E. Meloan: Chemical Separation, principles, techniques, and experiments, John Wiley & Sons inc, New York, 1999.
  • S. Ahuja: Handbook of bioseparations, Academic press, California, 2000.

Additional material:

—

  Course activities and grading method

Lectures:

0 points (4 hours a week)

Syllabus:

  • Sample preparation.
  • Extraction.
  • Liquid-liquid, liquid-solid and solid-liquid extraction.
  • Solid phase extraction.
  • Supercritical extraction.
  • Headspace technique.
  • Ion exchange.
  • Separation methods based on different principles of chromatography.
  • Optimization of chromatographic separation.
  • Electrophoresis.
  • Capillary electrophoresis.
  • Isoelectric focusing.
  • Dialysis.
  • Application of mass spectrometry in the analysis of biomolecules.
  • The effect of applied detectors on the efficiency of a separation method.
  • Selection of the suitable separation method for the quantitative analysis of various real samples.

Labwork:

20 points (2 hours a week)

Semester papers:

20 points

Oral exam:

60 points