Course code:
312A1		 Course name:
Separation Methods

Academic year:

2019/2020.

Attendance requirements:

302H1 / 302A1 / 301B1

ECTS:

6

Study level:

basic academic studies, integrated basic and graduate academic studies

Study programs:

Chemistry: 3. year, summer semester, elective (E13H1) course

Biochemistry: 3. year, summer semester, elective (E13B1) course

Chemical Education: 3. year, summer semester, elective (E13P1) course

Teachers:

Rada M. Baošić, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Maja M. Natić, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: four hours of lectures (4+0+0)

Goals:

Within this course students are introduced to the basic principles of separation methods and the ways of selecting the appropriate method depending on the needs.

Outcome:

A student is able to select and apply the most suitable method for the appropriate separation.

Teaching methods:

Lectures, term paper.

Extracurricular activities:

—

Coursebooks:

Main coursebooks:

  • C. E. Meloan: Chemical Separation, principles, techniques, and experiments, John Wiley & Sons inc, New York, 1999.
  • S. Ahuja: Handbook of bioseparations, Academic press, California, 2000.

Supplementary coursebooks:

  • M. Radojevic, V. Baskhin: Practical environmental analysis, The Royal Society of Chemistry, Cambridge, UK, 1999.

Additional material:

—

  Course activities and grading method

Lectures:

10 points (4 hours a week)

Syllabus:

Sample preparation. Extraction. Liquid-liquid, liquid-solid and solid-liquid extraction. Solid phase extraction. Supercritical extraction. Headspace technique. Ion exchange. Separation methods based on different principles of chromatography. Optimization of chromatographic separation. Electrophoresis. Capillary electrophoresis. Isoelectric focusing. Dialysis. Application of mass spectrometry in the analysis of biomolecules. The effect of applied detectors on the efficiency of a separation method. Selection of the suitable separation method for the quantitative analysis of various real samples.

Semester papers:

30 points

Oral exam:

60 points