Course code:
355H2		 Course name:
Electrochemical Nano-bio-sensors

Academic year:

2023/2024.

Attendance requirements:

There are no requirements.

ECTS:

9

Study level:

graduate academic studies, integrated basic and graduate academic studies

Study programs:

Chemical Education: 5. year, winter semester, elective (E5AP2) course

Chemistry: 1. year, winter semester, elective (E52H2) course

Teacher:

Dalibor M. Stanković, Ph.D.
assistant professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistant:

Slađana Z. Đurđić, Ph.D.
assistant with PhD, Faculty of Chemistry, Studentski trg 12-16, Beograd

Hours of instruction:

Weekly: four hours of lectures + two hours of exercises + three hours of labwork (4+2+3)

Goals:

The aim of this course is to introduce students to the possibilities of practical use of electrochemistry in various fields of research. The course is divided into thematic areas and allows students to gain insight into the potential use of modern electrochemical methods in clarifying the results of their research. A separate section will relate to specific interactions in living systems and the environment as well as the development of sensors and biosensors to monitor them. In addition, students will be introduced to the latest third generation of biosensors: modes of synthesis of mediators (organic compounds and new materials) and recombination and adaptation of enzymes required for their development and construction. At the end of the course students will be introduced to the possibilities of combining electrochemical methods for different techniques in order to obtain better results and to explain specific phenomena.

Outcome:

Upon completion of this course, students will be able to independently and team prepare experiments and select electrochemical methods, as well as successfully combine these methods with other experimental techniques to complement their further research or find answers and explanations for particular parts of their research.

Teaching methods:

Lectures, labwork, semmester papers.

Extracurricular activities:

Writing semmester papers.

Coursebooks:
  1. °. Bard, Martin Stratmann: Encyclopedia of Electrochemistry
  2. H. Girault: Analytical and physical electrochemistry
  3. Springer Series on Chemical Sensors and Biosensors: Methods and Applications

Additional material:

—

  Course activities and grading method

Lectures:

10 points (4 hours a week)

Syllabus:

  1. Introduction to modern electrochemical methods.
  2. Instrumentation and electroanalytical chemistry.
  3. Inorganic electrochemistry, monitoring and interactions.
  4. Organic electrochemistry, monitoring and interactions.
  5. Bioelectrochemistry, monitoring and interactions.
  6. Electrochemical degradations.
  7. Modified electrodes.
  8. Electrochemical sensors and biosensors.
  9. Gas (bio)sensors, (bio)sensors in environmental protection, (bio)sensors in medicine, (bio)sensors in industry.
  10. Types of mediators in biosensor construction: organic compounds, polymer matrices, new materials and composites.
  11. Mathematical modeling of (bio) sensors; receptor and mediator design for the next generation of biosensors.
  12. Wearable and wireless biosensors.
  13. Electrochemical methods in combination with other techniques.

Exercises:

0 points (2 hours a week)

Labwork:

15 points (3 hours a week)

Syllabus:

  1. Introduction to electrochemical workstations software.
  2. Preparation of carbon paste and printed electrodes.
  3. Electrochemical characterization of complex compounds.
  4. Electrochemical characterization of biologically active compounds.
  5. Interactions of newly synthesized compounds with DNA, electrochemical and spectrophotometric, comparison of results.
  6. DNA biosensors.
  7. Development of an electrochemical method for the detection of environmental pollutants based on carbon paste electrode.
  8. Modification of electrodes.
  9. Literature review and selection of seminar paper topics based on master's and doctoral thesis topics.
  10. Construction of biosensor based on modified carbon paste electrode, method development.
  11. Design of biosensors based on printed electrode and complex materials as mediators.
  12. Use of new materials in electroanalytical chemistry.
  13. Selection of a suitable modifier / analyte system for the development of an electroanalytical method.
  14. Interactions in living systems.
  15. Defense of seminar papers.

Semester papers:

25 points

Oral exam:

50 points